PDF Laser Ablation and Desorption

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  2. Multimodal laser ablation/desorption imaging analysis of Zn and MMP in breast tissues.
  3. Laser ablation

Strittmatter, N. Characterization and identification of clinically relevant microorganisms using rapid evaporative ionization mass spectrometry. Cameron, S. Scientific Reports 6 , Bodai, Z. Hillenkamp, F. A high-sensitivity laser microprobe mass analyzer. In situ , real-time identification of biological tissues by ultraviolet and infrared laser desorption ionization mass spectrometry.

Analytical Chemistry 83 , — Fahy, E. RJLR Xia, J. MetaboAnalyst 3. Temelkuran, B. Wavelength-scalable hollow optical fibres with large photonic bandgaps for CO2 laser transmission. Nature , — Pedregosa, F. Scikit-learn: Machine learning in Python. The Journal of Machine Learning Research 12 , — Shannon, P. Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Research 13 , — Download references. The authors would like to thank Imperial College Healthcare Trust for providing isolates and for their technical input.

Study was planned by S. Experiments were conducted by S. Data was analysed and interpreted by S. The manuscript was written by S. All authors have given approval to the final version of the manuscript. Neither funder had input into initial study design nor on decision of when and where to publish this manuscript. This paper does not promote any commercially available product of the Waters Corporation. No other competing interests, financial or otherwise, are declared by the other authors. Reprints and Permissions. By submitting a comment you agree to abide by our Terms and Community Guidelines.

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Advanced search. Skip to main content. Subjects Clinical microbiology Mass spectrometry. Abstract The accurate and timely identification of the causative organism of infection is important in ensuring the optimum treatment regimen is prescribed for a patient. Introduction The rapid and accurate identification of the causative organism of an infection is fundamental in ensuring that a patient receives the most appropriate and effective treatment, in the shortest possible timeframe.

Figure 1. Full size image. Results and Discussion The use of REIMS as a potential tool in diagnostic microbiology has, to date, utilised electrical diathermy to thermally disrupt biomass, creating a vapour containing analytes in gas-phase. Optimisation of ALDI-MS Analytical Set-Up Initial optimisation of the laser operational parameters, including power and pulse modes was conducted on 15 Escherichia coli isolates using the experimental set-up shown in Fig. Figure 2. Figure 3.

Figure 4. Figure 5. Full size table. Experimental Section Culturing of Microorganisms For REIMS analysis, a total of microbial isolates 15 isolates collected from distinct clinical patients from ten microbial species — nine bacterial and one yeast were cultured according to conditions outlined in the accompanying supporting information. Data and Statistical Analysis To correct for mass drift and remove background mass spectral signals, all.

Safety Considerations Throughout the work detailed here, all microbial isolates were treated as potential Hazard Group 2 organisms and thus, were handled within containment level two facilities. References 1. Article Google Scholar 6.

  1. Introduction;
  2. Magmatic Sulfide Deposits: Geology, Geochemistry and Exploration.
  3. Laser ablation and desorption from calcite from ultraviolet to mid-infrared wavelengths..

Article Google Scholar Acknowledgements The authors would like to thank Imperial College Healthcare Trust for providing isolates and for their technical input. Author information Author notes Simon J.

Multimodal laser ablation/desorption imaging analysis of Zn and MMP in breast tissues.

Cameron and Zsolt Bodai contributed equally. Supplementary information Supplementary Information. About this article. Comments By submitting a comment you agree to abide by our Terms and Community Guidelines. Download PDF. Click here to close this overlay, or press the "Escape" key on your keyboard. The Japan Society of Applied Physics JSAP serves as an academic interface between science and engineering and an interactive platform for academia and the industry. JSAP is a "conduit" for the transfer of fundamental concepts to the industry for development and technological applications.

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